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牙周膜干细胞表面标记物

细胞储存应用 2023年05月14日 16:04 105 im

  

Introduction

  The periodontal ligament (PDL) is a thin layer of connective tissue that surrounds the teeth and anchors them to the surrounding bone. The PDL contains various cell types, including fibroblasts, osteoblasts, and stem cells. Among these, PDL stem cells are of particular interest due to their potential therapeutic applications. These cells can differentiate into various cell types, such as osteoblasts, chondrocytes, adipocytes, and neural cells, depending on the environmental cues.

  

Surface markers of PDL stem cells

  PDL stem cells express various surface markers that help in their isolation and characterization. Some of the commonly used surface markers for PDL stem cells are:

  

  1. CD90: This marker is also known as Thy-1, and it is a glycoprotein that is highly expressed in mesenchymal stem cells, including PDL stem cells. CD90 is involved in cell-cell and cell-matrix interactions and plays a crucial role in cell migration and proliferation.
  2. CD73: This marker is also called ecto-5-nucleotidase, and it is a surface enzyme that converts extracellular nucleotides into adenosine, which has anti-inflammatory and immunosuppressive effects. CD73 is expressed in various cell types, including PDL stem cells, and is used as a marker for identifying mesenchymal stem cells.
  3. CD146: This marker is also known as melanoma cell adhesion molecule (MCAM), and it is a cell adhesion molecule that is important for maintaining vascular integrity and regulating angiogenesis. CD146 is expressed in pericytes, which are multipotent stem cells that reside in the blood vessels. PDL stem cells also express CD146, and it is used as a marker for identifying pericytes.
  4. STRO-1: This marker is a cell surface glycoprotein that was originally identified in bone marrow stromal cells. STRO-1 is used as a marker for identifying mesenchymal stem cells, including PDL stem cells. It is involved in cell adhesion, migration, and proliferation.

Potential therapeutic applications of PDL stem cells

  PDL stem cells have immense potential for regenerative medicine due to their ability to differentiate into various cell types and secrete growth factors and cytokines that promote tissue repair and regeneration. Some of the potential therapeutic applications of PDL stem cells are:

  

  1. Dental tissue regeneration: PDL stem cells can differentiate into cementoblasts, which are the cells responsible for forming the cementum that covers the root surface of the teeth. PDL stem cells can be used for regenerating the periodontal tissues that are lost due to gum disease or trauma.
  2. Bone regeneration: PDL stem cells can differentiate into osteoblasts, which are the cells responsible for forming new bone tissue. PDL stem cells can be used for regenerating bone tissue that is lost due to fractures, osteoporosis, or other bone diseases.
  3. Cartilage regeneration: PDL stem cells can differentiate into chondrocytes, which are the cells responsible for forming cartilage tissue. PDL stem cells can be used for regenerating cartilage tissue that is lost due to arthritis or other joint disorders.
  4. Neuroregeneration: PDL stem cells can differentiate into neural cells, including neurons and glial cells, and can be used for repairing the damaged nervous system.

Conclusion

  PDL stem cells are a promising source of stem cells for regenerative medicine. They express various surface markers that help in their isolation and characterization. PDL stem cells have the potential to differentiate into various cell types and promote tissue repair and regeneration. Further research is needed to fully understand the therapeutic potential of PDL stem cells and to develop safe and effective treatments using these cells.

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