胚胎干细胞成功扩展
细胞储存费用的比较分析
2023年05月24日 14:17 63
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1. Introduction
Embryonic stem cells (ESCs) are a type of pluripotent cell that has the ability to develop into every cell type found in the body. This has the potential to revolutionize medicine by allowing for personalized regenerative therapies, where damaged or diseased tissues can be replaced with healthy ones grown from a patients own cells. However, the use of ESCs in research has been controversial due to ethical concerns about their source: embryos left over from in vitro fertilization clinics.
To circumvent this controversy, researchers have been working to develop methods to generate induced pluripotent stem cells (iPSCs) from adult cells, which are reprogrammed to have similar characteristics as ESCs. However, iPSCs have limitations, including an increased risk of genetic mutations and cell senescence.
Therefore, the successful expansion of ESCs without the need for fresh human embryos would represent an important breakthrough in stem cell research and regenerative medicine.
2. Background
For over a decade, scientists have been trying to develop methods to maintain and expand human ESCs without using animal-based feeder cells. Feeder cells are used to provide a supportive microenvironment for the growth and development of ESCs, creating an ethical dilemma due to the unpredictability of animal products in terms of zoonosis, viral contamination, and animal welfare.
In 2013, researchers at the University of Wisconsin-Madison successfully derived human ESCs from a single blastomere—a single cell isolated from an eight-cell embryo—using a defined culture system that didnt include animal-derived products. Although this was a major breakthrough, it represented a largely experimental advance and the system could only support limited long-term expansion of the ESCs.
Recently, a research team at the Hebrew University of Jerusalem developed a new culture method that allowed for the expansion of human ESCs for over a year without using feeder cells or animal products. Their study was published in the journal Cell Stem Cell in June 2020.
3. Methods
The researchers used an alginate hydrogel to provide a three-dimensional culture environment for human ESCs, which improved their viability and increased their proliferation rates. They also used conditioned media—a solution containing secreted factors from other cells—to mimic the supportive effect of feeder cells without the accompanying ethical concerns. The team optimized the components of the culture system and demonstrated its long-term efficacy by expanding several different lines of ESCs for over a year while maintaining their pluripotency and genetic stability.
4. Significance
This study marks a major milestone in stem cell research, as it provides a reliable and ethical method for the expansion of human ESCs without the use of feeder cells or animal products. This development has immense potential for medical applications, especially in regenerative medicine.
The use of feeder-free and xeno-free culture systems is the future direction of inducing pluripotent stem cell technology. It reduces the risk of contamination when these cells are implanted or used for transplantations, and facilitating the long-term expansion of high-quality human ESCs paves the way for their clinical use.
While this study is important, further studies need to be done in order to optimize the methods for generating human stem cells with specific tissue regenerative abilities. Nonetheless, this research advances the ability to generate patient- and disease-specific cells that serve as first steps towards developing personalized therapies.
5. Conclusion
In conclusion, the successful expansion of human ESCs without the use of feeder cells or animal products represents a major breakthrough in stem cell research and regenerative medicine. While the technology still needs to be optimized, its potential applications are vast and could lead to personalized treatment options for many diseases.
This study shows that ethical considerations can be incorporated into the development of scientific research. It is possible to create a better and more ethical build of science that benefits everyone.
2. Background
For over a decade, scientists have been trying to develop methods to maintain and expand human ESCs without using animal-based feeder cells. Feeder cells are used to provide a supportive microenvironment for the growth and development of ESCs, creating an ethical dilemma due to the unpredictability of animal products in terms of zoonosis, viral contamination, and animal welfare.
In 2013, researchers at the University of Wisconsin-Madison successfully derived human ESCs from a single blastomere—a single cell isolated from an eight-cell embryo—using a defined culture system that didnt include animal-derived products. Although this was a major breakthrough, it represented a largely experimental advance and the system could only support limited long-term expansion of the ESCs.
Recently, a research team at the Hebrew University of Jerusalem developed a new culture method that allowed for the expansion of human ESCs for over a year without using feeder cells or animal products. Their study was published in the journal Cell Stem Cell in June 2020.
3. Methods
The researchers used an alginate hydrogel to provide a three-dimensional culture environment for human ESCs, which improved their viability and increased their proliferation rates. They also used conditioned media—a solution containing secreted factors from other cells—to mimic the supportive effect of feeder cells without the accompanying ethical concerns. The team optimized the components of the culture system and demonstrated its long-term efficacy by expanding several different lines of ESCs for over a year while maintaining their pluripotency and genetic stability.
4. Significance
This study marks a major milestone in stem cell research, as it provides a reliable and ethical method for the expansion of human ESCs without the use of feeder cells or animal products. This development has immense potential for medical applications, especially in regenerative medicine.
The use of feeder-free and xeno-free culture systems is the future direction of inducing pluripotent stem cell technology. It reduces the risk of contamination when these cells are implanted or used for transplantations, and facilitating the long-term expansion of high-quality human ESCs paves the way for their clinical use.
While this study is important, further studies need to be done in order to optimize the methods for generating human stem cells with specific tissue regenerative abilities. Nonetheless, this research advances the ability to generate patient- and disease-specific cells that serve as first steps towards developing personalized therapies.
5. Conclusion
In conclusion, the successful expansion of human ESCs without the use of feeder cells or animal products represents a major breakthrough in stem cell research and regenerative medicine. While the technology still needs to be optimized, its potential applications are vast and could lead to personalized treatment options for many diseases.
This study shows that ethical considerations can be incorporated into the development of scientific research. It is possible to create a better and more ethical build of science that benefits everyone.
3. Methods
The researchers used an alginate hydrogel to provide a three-dimensional culture environment for human ESCs, which improved their viability and increased their proliferation rates. They also used conditioned media—a solution containing secreted factors from other cells—to mimic the supportive effect of feeder cells without the accompanying ethical concerns. The team optimized the components of the culture system and demonstrated its long-term efficacy by expanding several different lines of ESCs for over a year while maintaining their pluripotency and genetic stability.
4. Significance
This study marks a major milestone in stem cell research, as it provides a reliable and ethical method for the expansion of human ESCs without the use of feeder cells or animal products. This development has immense potential for medical applications, especially in regenerative medicine.
The use of feeder-free and xeno-free culture systems is the future direction of inducing pluripotent stem cell technology. It reduces the risk of contamination when these cells are implanted or used for transplantations, and facilitating the long-term expansion of high-quality human ESCs paves the way for their clinical use.
While this study is important, further studies need to be done in order to optimize the methods for generating human stem cells with specific tissue regenerative abilities. Nonetheless, this research advances the ability to generate patient- and disease-specific cells that serve as first steps towards developing personalized therapies.
5. Conclusion
In conclusion, the successful expansion of human ESCs without the use of feeder cells or animal products represents a major breakthrough in stem cell research and regenerative medicine. While the technology still needs to be optimized, its potential applications are vast and could lead to personalized treatment options for many diseases.
This study shows that ethical considerations can be incorporated into the development of scientific research. It is possible to create a better and more ethical build of science that benefits everyone.
4. Significance
This study marks a major milestone in stem cell research, as it provides a reliable and ethical method for the expansion of human ESCs without the use of feeder cells or animal products. This development has immense potential for medical applications, especially in regenerative medicine.
The use of feeder-free and xeno-free culture systems is the future direction of inducing pluripotent stem cell technology. It reduces the risk of contamination when these cells are implanted or used for transplantations, and facilitating the long-term expansion of high-quality human ESCs paves the way for their clinical use.
While this study is important, further studies need to be done in order to optimize the methods for generating human stem cells with specific tissue regenerative abilities. Nonetheless, this research advances the ability to generate patient- and disease-specific cells that serve as first steps towards developing personalized therapies.
5. Conclusion
In conclusion, the successful expansion of human ESCs without the use of feeder cells or animal products represents a major breakthrough in stem cell research and regenerative medicine. While the technology still needs to be optimized, its potential applications are vast and could lead to personalized treatment options for many diseases.
This study shows that ethical considerations can be incorporated into the development of scientific research. It is possible to create a better and more ethical build of science that benefits everyone.
5. Conclusion
In conclusion, the successful expansion of human ESCs without the use of feeder cells or animal products represents a major breakthrough in stem cell research and regenerative medicine. While the technology still needs to be optimized, its potential applications are vast and could lead to personalized treatment options for many diseases.
This study shows that ethical considerations can be incorporated into the development of scientific research. It is possible to create a better and more ethical build of science that benefits everyone.
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